The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the protocol to make 1 liter of 10x concentrate PBS. Combine the following:

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The reaction given:

H2PO4-(aq) ⇆ H+(aq) + HPO42-( aq)

Here, H2PO4- ion serves as a proton donor, while HPO42- ion is a proton acceptor. In a PBS solution, Na2HPO4 dissociates into Na+ and HPO42-. As a result, this molecule serves as a proton acceptor. In contrast, KH2PO4 dissociates into K+ and H2PO4-. As H2PO4- is a proton donor, KH2PO4 serves as a proton donor in a PBS solution.

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Question 8: If you had the choice between a 1 liter beaker and a 1 liter graduated cylinder, which one should you use to measure volumes with maximal precision when making 1 liter of PBS?

the cup should never be used for accuracy because its marks are approximate and not accurate.

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Question 11: The PBS protocol above says to adjust pH to 7.4 with HCl. What does this imply on the pH of 10x PBS before adjusting the pH, would it be greater or smaller than 7.4?

pH scale indicates the acidity and alkalinity of a solution. pH is used to show if the solution is acidic or basic and reflects the concentration of H+ ion. pH...

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Question 12: The last step in the protocol is to autoclave the 10x PBS solution. Why do you think this step is important? Look up the definition of autoclave if you are unsure what it means.

Autoclave is used to sterilise solutions using high temperature and pressure. As PBS is used in various assays (immunocytochemical and immunofluorescent staining, cell culture, protein purification, etc.), it is important...

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What is the mass in grams of KCl present in23.0 g KCl of 12.50 KCl solution?

If mass percent of KCl is 23% 0.23 x 12.5 = 2.875 .

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Fill in the appropriate unit conversions. (Report the molar mass of KCl using three sig. figs.) ? mol/L​ = 5.00 g KCl/100. mL​×1 mol KCl/ KCl​×/1 L ​

Molar mass of KCl = 74.6g/mol

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The ksp of magnesium phosphate is 1 x 10 -24 at 25oC. If the magnesium phosphate is dissolved in 250. mL of water. How many grams of magnesium phosphate were dissolved (think about multiple units)

Mass of Magnesium phosphate =62.7g

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Calculate the molarity of the NaCl NaCl solution when 14.6 g g of NaCl NaCl is dissolved in 100 mL mL of water.

Molar mass of NaCl= 58.4414.6/58.44=0.25mol0.25/0.1= 2.5mol/L

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What is the concentration (in mol / dm3) of KCl in the solution formed by mixing 25 mL of KCl solution, 0.100 M concentration with 50 mL of KCl solution, 0.100 M concentration?

The concentration of both solutions is equal. Therefore, we will get 75 ml of 0.100 M KCl solution.0.100 M = 0.100 mol/dm3.

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When heated, KClO3 decomposes into KCl and O2. 2 KClO3 ⟶ 2 KCl + 3 O2 If this reaction produced 53.7 g KCl, how many grams of O2 were produced?

The molar mass of potassium chloride is 39+35.5 = 74.5. Thus formed 53.7/74.5 = 0.72 mol oxygen. Based on the oxygen reaction equation formed (0.72*2)/3 = 0.48 mol or 0.48*22.4...

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The PBS protocol above says to adjust pH to 7.4 with HCl. What does this imply on the pH of 10x PBS before adjusting the pH, would it be greater or smaller than 7.4?

 PBS stands for phosphate buffered saline which is used in biological research. It is a salty solution that contains sodium chloride, sodium phosphate, potassium chloride and potassium phosphate. The buffer...

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