Question 8: If you had the choice between a 1 liter beaker and a 1 liter graduated cylinder, which one should you use to measure volumes with maximal precision when making 1 liter of PBS?

Md Ariful Islam

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Md Ariful Islam

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the cup should never be used for accuracy because its marks are approximate and not accurate.

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Question 11: The PBS protocol above says to adjust pH to 7.4 with HCl. What does this imply on the pH of 10x PBS before adjusting the pH, would it be greater or smaller than 7.4?

pH scale indicates the acidity and alkalinity of a solution. pH is used to show if the solution is acidic or basic and reflects the concentration of H+ ion. pH...

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Question 12: The last step in the protocol is to autoclave the 10x PBS solution. Why do you think this step is important? Look up the definition of autoclave if you are unsure what it means.

Autoclave is used to sterilise solutions using high temperature and pressure. As PBS is used in various assays (immunocytochemical and immunofluorescent staining, cell culture, protein purification, etc.), it is important...

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What is precision agriculture and why is it important practice precision agriculture?

Precision techniques allow farmers to apply fertilizers to specific areas and can allow farmers to prescribe applications based on nutrient needs in a specific area.

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An 8 gram metal object displaces 2 mL of water in a graduated cylinder. What is the density of the metal object?

d=m/V=8/2=4(g/ml)=4(g/cm3)

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The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the protocol to make 1 liter of 10x concentrate PBS. Combine the following:

The reaction given:H2PO4-(aq) ⇆ H+(aq) + HPO42-( aq)Here, H2PO4- ion serves as a proton donor, while HPO42- ion is a proton acceptor. In a PBS solution, Na2HPO4 dissociates into Na+ and HPO42-. As a result, this molecule...

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A 75.0 g rock is dropped into a graduated cylinder containing 75.0 mL of water. After the rock is dropped in, the water level rises to 78.1 mL. What is the density of the rock?

78.1-75= 3.1D = M/V= 75/3.1 = 24.194g/ml

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An empty graduated cylinder weighs 75.0g. When sodium hydroxide is added the mass of both of 145.3. The density of the hydroxide is 2.4 g/,ml. What volume of sodium was added?

Volume of sodium=29.29ml

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When exactly 50% of a 1.4 M solution is poured into a beaker , what is the concentration of the solution in the beaker (in M)?

Concentration of the solution in the beaker =1.43M

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Why must you pour KIO[sub]3[/sub] into a beaker from the volumetric flask instead directly into the beaker oh NaHSO[sub]3[/sub].

Probably, you mean the clock reaction. In this reaction amount of KIO3 is crucial. So, we need firstly to pour solution of KIO3 into the volumetric flask and then, well-known...

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which apparatus is used prepare solution with certain concentrations? 1.Graduated cylinder 2. Volumetric flask 3.Beaker 4. Erlenmeyder 5.Burette

If the solute is a solid, you would use a laboratory balance to measure out the mass. Measure the liquid solvent with a graduated cylinder. Combine them in a flask....

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