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Ion-exchange chromatography is a method that can be used to purify proteins on the basis of _____.
1. size 2. hydrophobicity 3. charge 4. primary structure
Ion-exchange chromatography is a method that can be used to purify proteins on the basis of charge (3).
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Chromatography was first used in what year
Russian botanist Mikhail Tsvet invented column chromatography in 1906
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When potassium metal is added to water, it react violently, but when potassium react with methanol, it forms bubbles. Why is this happening? why is potassium more reactive in water than in methanol
The molten metal spreads over the water and exposes a larger surface to water. Also, the hydrated radius of lithium is the greatest out of all alkali metals. This reduces...
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If a chemist is going to prepare a chromatography using diethyl ether and ethanol. Which solvent would they use to start the elution? What would happen if they used the other instead?
Solution.Diethyl ether and ethanol chromatography is a development-displacement chromatography. Diethyl ether is the developer, and ethanol is the displacer. Therefore, to begin with, the column is filled with the test...
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Methanol is burned in a bomb calorimeter. Liquid water is formed as a product. If 34.0 g of methanol reacts, what is the expected temperature change in a calorimeter with a heat capacity of 6.75 kJ/C
2CH3OH+ 3O2 = 2CO2 + 4H2O ∆H = 4(-285.8 kJ/mol) + 2(-393.510 kJ/mol) ndash; (2(-201.0 kJ/mol)) = -1528.22 kJ/mol n(CH3OH) = 34 g / 32g/mol = 1.0625mol Q = ∆H*n...
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dear sir
I HAVE A QUESTION ABOUT GAS CHROMATOGRAPHY.WHEN WE USE CONSTANT PRESSURE OR CONSTANT FLOW FOR COLUMN OR IN MAKE METHOD FOR ANALYSIS?
BEST REGARDS
TONE
If you're doing a relatively minor temperature program (say from 50C to 150C), then you won't notice much difference between constant flow and constant pressure modes. If you're doing a...
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What is the principle of ion exchange chromatography?Write any one application of this chromatography.
Ion exchange chromatography allows the separation of molecules based on ionic interactions. The stationary phase has charged functional groups that interact with the analyzed ionized molecules of opposite charge. This...
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methanol is burned in a camp stove to provide heat for cooking if 18 g of methanol is burned what will the volume of oxygen measured at 37°C and 98.8 KPA required for complete combustion
2CH3OH + 3O2 = 2CO2 + 4H2On (O2) = 3 x n (CH3OH) /2M (CH3OH) = 32.04 g/moln (CH3OH) = 18 / 32.04 = 0.56 moln (O2) = 3 x...
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An unknown liquid was found to have a density of 69.22lb/ft3. The density of ethylene glycol (the liquid used in antifreeze)is 1.1088 g/mL. Is the unknown liquid ethylene glycol?
69.22lb/ft3=1.1088 g/mL.Therefore the unknown liquid is ethylene glycol.
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The hydronium content of a popular soft drink is measured and found to be 5.63×10⁻³ M. What is the pH of this soft drink?
Solution:[H3O+] = 5.63×10-3 MThe pH is calculated using the expression:pH = - log[H3O+]pH = - log(5.63×10-3) = 2.2495 = 2.25pH = 2.25 Answer: The pH of this soft drink is...
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