Why is Phosphofructokinase-1 reaction considered the key regulatory step in Glycolysis and not Hexokinase reaction?

Autoclave is used to sterilise solutions using high temperature and pressure. As PBS is used in various assays (immunocytochemical and immunofluorescent staining, cell culture, protein purification, etc.), it is important...

A promoter, the correct translation initiation sequence such as a ribosomal binding site and start codon, a termination codon, and a transcription termination sequence.

Resources for glycolysis: most monosaccharides, such as fructose, glucose, and galactose At the end of glycolysis, each molecule of glucose has yielded 2 molecules of pyruvate, 2 molecules of NADH,...

As the concentration of glucose in a cell equals approximately 1 mM, the ratio of glucose/hexokinase is 1 mM/0.02 mM = 50 : 1. As a result, one molecule of...

The enzyme that catalyzes the first step of glycolysis is Phosphofructokinase (PFK), which converts fructose-6-phosphate to fructose-1,6-bisphosphate. The two initial phosphorylations by hexokinase and PFK require ATP and are therefore irreversible. PFK is one...

Prokaryotic transcription and translation occur simultaneously in the cytoplasm, and regulation occurs at the transcriptional level. Eukaryotic gene expression is regulated during transcription and RNA processing, which take place in...

A carboxyl group is removed from pyruvate and released as carbon dioxide

The difference between aerobic glycolysis and anaerobic glycolysis is that aerobic glycolysis proceeds in the presence of oxygen and occurs in eukaryotic cells while anaerobic glycolysis proceeds in the absence of...

Glucokinase and Hexokinase are enzymes which phosphorylate glucose to glucose-6-phosphate, trapping glucose inside the cell.Compared to hexokinase, glucokinase has a higher Km (lower binding affinity) and a higher Vmax (increased...

In chemical kinetics, the rate of a reaction mechanism with several steps is often determined by the slowest step, known as the rate-determining step or rate-limiting step. In a reaction...