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Physical agents: temperature (high temperature and dry heat have the best effect), radiation (X-rays or UV-rays can induce mutations which cause imbalance in the vital processes), moist pressure (causes coagulation of proteins); Chemical agents: alcohols (cause denaturation of proteins and dissolve lipids), surface-active agents (emulsify fats), quaternary ammonium compounds (disrupt plasma membrane, and the cytoplasm leaks out).
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