The particles that are containes in the gel interrupt the path of the DNA fragments when they are attracted by the electric current, so the longest ones are the ones...
1 Answers 1 viewsThere exist an enzyme, called restriction enzyme, that can identify a particular nucleotide sequence, called restriction sites, and perform cleaving operation. This process separates genetic material into smaller fragments which...
1 Answers 1 viewsAs you know, gel uses electricity to separate bands of macro-molecular material by weight. You'd start by running a control gel. Let's say your gene codes for some macro-molecule...
1 Answers 1 viewsIt depends on what you are sequencing: if you are talking LARGE fragments, then an Agarose gel is the best option. You can adjust the size of the pores in...
1 Answers 1 viewsA low density of agarose is used to separate large fragments, whereas a high density is used to separate smaller fragments. However, when two fragments are of similar size,...
1 Answers 1 viewsis an effect of electric charge on particles across a particular volume of liquid and pore size (the gel). If the control does not have the same dimensions, the...
1 Answers 1 viewsEthidium bromide is used in the gel to view the DNA. Ethidium bromide is an intercalating agent that fits into the middle of the DNA molecule, causing it...
1 Answers 1 viewsis done after amplifying DNA samples through Polymerase Chain Reaction (PCR). However, the targeted gene for your DNA samples cannot be validated only by observing with your naked eye...
1 Answers 1 viewsThe electrophoresis is used in the separation of charged molecules according to sizes such as DNA, RNA, in a solution. The charged molecules move towards the respective poles and finally...
1 Answers 1 viewscan be used for the separation of DNA, RNA, and . For an in-depth look at the separation of proteins by gel electrophoresis please see -
1 Answers 1 views