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What is the purpose of a restriction enzyme in an electrophoresis gel?

Md Ariful Islam

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Md Ariful Islam

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There exist an enzyme, called restriction enzyme, that can identify a particular nucleotide sequence, called restriction sites, and perform cleaving operation. This process separates genetic material into smaller fragments which may contain gene(s) of interest. Information on the size of the fragment can be achieved through the use of gel electrophoresis.

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If you have a restriction enzyme that cuts a piece of DNA at two recognition sites, how many DNA fragments would you see on a gel?

YY is your restriction site. ABCD-YY-ABCDE-YY-ABCDEF When you cut, you'd get ABCD-Y Y-ABCDE-Y Y-ABCDEF

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What role does gel electrophoresis play in the study of DNA?

The particles that are containes in the gel interrupt the path of the DNA fragments when they are attracted by the electric current, so the longest ones are the ones...

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How could gel electrophoresis be applied to the identification of a gene mutation?

As you know, gel uses electricity to separate bands of macro-molecular material by weight. You'd start by running a control gel. Let's say your gene codes for some macro-molecule...

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What is the primary reason that DNA gel electrophoresis is usually undertaken using an agarose gel and not an acrylamide based gel as in protein electrophoresis?

It depends on what you are sequencing: if you are talking LARGE fragments, then an Agarose gel is the best option. You can adjust the size of the pores in...

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Does increasing or decreasing the density of agarose gel in electrophoresis separate similar sized fragments?

A low density of agarose is used to separate large fragments, whereas a high density is used to separate smaller fragments. However, when two fragments are of similar size,...

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In Gel Electrophoresis, why does volume of your control have to be the same as your test solution?

is an effect of electric charge on particles across a particular volume of liquid and pore size (the gel). If the control does not have the same dimensions, the...

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Why is gel electrophoresis used after pcr?

Ethidium bromide is used in the gel to view the DNA. Ethidium bromide is an intercalating agent that fits into the middle of the DNA molecule, causing it...

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Why is gel electrophoresis performed?

is done after amplifying DNA samples through Polymerase Chain Reaction (PCR). However, the targeted gene for your DNA samples cannot be validated only by observing with your naked eye...

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Why does gel electrophoresis work?

The electrophoresis is used in the separation of charged molecules according to sizes such as DNA, RNA, in a solution. The charged molecules move towards the respective poles and finally...

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Why do biologists use gel electrophoresis?

This process is used by molecular biologists to : 1) separate a mixture of DNA and RNA fragments by length. 2) estimate the size of DNA and RNA fragments....

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