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Why must you use the same restriction enzyme on the cell dna and on the plasmid?
Restriction endonuclease identifies and cuts the same pallindromic sequence in both DNA and Vector due to which when they will be mixed , their complementary bases will join and it...
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How do restriction enzyme cut at specific sequences?
The cuts are always made at specific nucleotide sequences . Different restriction enzymes recognise and cut different DNA sequences. Restriction enzymes work by shape to shape matching . It...
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What is a biological function of restriction enzyme?
act like a primitive immune system for some . They recognize a palindromic sequence of DNA (one that reads the same forward and backward.....like the name HANNAH) and the...
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How does a restriction enzyme limit or restrict the effect of a virus on a bacterial cell?
restrict foreign DNA by cutting specific palindromic sequences of DNA (palindromic like the name, HANNAH - reads the same both directions). These target sequences are usually 6 nucleotides in...
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What is the purpose of a restriction enzyme in an electrophoresis gel?
There exist an enzyme, called restriction enzyme, that can identify a particular nucleotide sequence, called restriction sites, and perform cleaving operation. This process separates genetic material into smaller fragments which...
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What is the primary reason that DNA gel electrophoresis is usually undertaken using an agarose gel and not an acrylamide based gel as in protein electrophoresis?
It depends on what you are sequencing: if you are talking LARGE fragments, then an Agarose gel is the best option. You can adjust the size of the pores in...
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Does increasing or decreasing the density of agarose gel in electrophoresis separate similar sized fragments?
A low density of agarose is used to separate large fragments, whereas a high density is used to separate smaller fragments. However, when two fragments are of similar size,...
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A piece of wood that measures 3.0 cm by 6.0 cm by 4.0 cm has a mass of 80.0 grams. What the density of the wood? Would the piece of wood float in water?
The density of a substance can be calculated using: $d=m/V$ where $m$ is the mass, and $V$ is the volume. Here we do not have the volume given, however...
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A piece of metal that measures 33.0 cm by 46.0 cm by 48.0 cm has a mass of 800.0 grams. What is the density of the metal? Would the piece of metal float in water?
By definition, $rho_"metal"="mass of metal"/"volume of metal"$ $=(800*g)/(33.0*cmxx46.0*cmxx48.0*cm)=0.0659*g*cm^-3$ And this is an IMPROBABLY undense metal. The LEAST dense metal, lithium, is approx. $0.5*g*cm^-3$...(at any rate this metal reacts with water)...the...
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Lewis has a copper pipe with a length of 150 cm and a mass of 800 grams. He cuts a piece of the copper pipe with a length of 90 cm. How do you work out the mass of this piece of copper pipe?
I would consider the Linear of the pipe as: $delta="Linear Density"="mass"/"length"$ or: $delta=800/150=5.3g/(cm)$ When you cut it you are left with $90cm$ of pipe but with the same linear density...
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