The particles that are containes in the gel interrupt the path of the DNA fragments when they are attracted by the electric current, so the longest ones are the ones...
There exist an enzyme, called restriction enzyme, that can identify a particular nucleotide sequence, called restriction sites, and perform cleaving operation. This process separates genetic material into smaller fragments which...
As you know, gel uses electricity to separate bands of macro-molecular material by weight. You'd start by running a control gel. Let's say your gene codes for some macro-molecule...
It depends on what you are sequencing: if you are talking LARGE fragments, then an Agarose gel is the best option. You can adjust the size of the pores in...
A low density of agarose is used to separate large fragments, whereas a high density is used to separate smaller fragments. However, when two fragments are of similar size,...
is an effect of electric charge on particles across a particular volume of liquid and pore size (the gel). If the control does not have the same dimensions, the...
Ethidium bromide is used in the gel to view the DNA. Ethidium bromide is an intercalating agent that fits into the middle of the DNA molecule, causing it...
is done after amplifying DNA samples through Polymerase Chain Reaction (PCR). However, the targeted gene for your DNA samples cannot be validated only by observing with your naked eye...
The electrophoresis is used in the separation of charged molecules according to sizes such as DNA, RNA, in a solution. The charged molecules move towards the respective poles and finally...
This process is used by molecular biologists to : 1) separate a mixture of DNA and RNA fragments by length. 2) estimate the size of DNA and RNA fragments....
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