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Blood typing (in general) involves the use of three different antibodies for three different antigens. These three antibodies are Anti-A, Anti-B and Anti-D and are usually coloured differently to avoid error in reading.

The method used is simple: a drop each of Anti-A, Anti-B and Anti-D is placed with the cavities of a cavity slide and one drop of blood each is mixed using different glass mixers (so as not to give erroneous readings).

Depending on which drop demonstrates coagulation or not, the blood type is determined.

If coagulation takes place in Anti-A and not in Anti-B, the blood group is A.
If coagulation takes place in Anti-B and not in Anti-A, the blood group is B.
If coagulation takes place in both Anti-A and in Anti-B, the blood group is AB.
If coagulation takes place in neither Anti-A nor Anti-B, the blood group is O.

If coagulation takes place in Anti-D, the blood subgroup is Rh+.
If coagulation does not take place in Anti-D, the blood subgroup is Rh-.

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