In which calluses did the new gene insert inside an existing corn gene

Md Ariful Islam

0 like 0 dislike
1 views
Share with your friends

1 Answers

Md Ariful Islam

Call

You can check your ligation products by gel electrophoresis or PCR using plasmid primers across the insert but the number of ligation products and their low concentration makes analysis by agarose gel electrophoresis an impractical method. So sequencing could be a better option.

0 like 0 dislike
1 views
Talk Doctor Online in Bissoy App
3.1 Describe the process of cloning a DNA fragment into the PstI site of the vector pBR322. How would you screen for clones that contain an insert?

At first, the DNA fragment and the vector pBR322 must be digested with the restriction enzyme PstI. Next, the digested fragment and the vector must be resolved in the agarose...

1 Answers 1 views
How would you verify that the plasmid contains your insert of interest, in the proper position and orientation?

Another option for determining insert orientation is to use a primer from within the insert and primers from the plasmid vector for PCR reactions. The two plasmid primers would be...

1 Answers 1 views
what is a change in the position of a gene or part of a gene called

Gene Mutation

1 Answers 1 views
why does the change in only one DNA base of a gene does not always result in a different protein being produced by the gene

CHANGE IN DNA BASE GENEAbstract: Base substitution involves swapping one nucleotide for another during DNA replication, base substitution can lead to three different sub-categories of mutation; missense, nonsense and silent...

1 Answers 1 views
If the corn has 25,500 kCal of energy available, how much energy is available for the python?

According to the energy pyramid concept, only 10% of received energy is transferred to the next trophic level while 90% of the energy is lost as heat. As a result,...

1 Answers 1 views
A homozygous tall corn plant is crossed with a heterozygous tall corn plant. a) which trait is dominant and how do you know?

The dominant trait is a tall, because it manifests itself in both a homozygous and a heterozygous state.

1 Answers 1 views
GENE F DNA: TAG TAG TCT mRNA: tRNA: AMINO ACID SEQUENCE: TRAIT: GENE G DNA: CCA TCC TTT GGG mRNA: tRNA: AMINO ACID SEQUENCE: TRAIT: GENE H DNA: CCA CAT AAT mRNA: tRNA: AMINO ACID SEQUENCE: TRAIT:

TAC/TTC/AAA/CCG/CGT/GGG/ATTmRNA code can be derived as follows. In RNA instead of Thymine (T) you have Uracil (U)mRNA string will have the complementary base pair to the DNA stringComplementary paring -A-T...

1 Answers 1 views
Which type of plant would be expected to suffer the most and the earliest during a drought? A. Aloe B. Corn C. Dandelion D. Crabgrass

B. Corn

1 Answers 1 views
the process of inspiration occurs as a)lungs pull air inside b) passive expansion of air take place c)passive transport of air take place d) air is pushed inside

a)lungs pull air inside

1 Answers 1 views
Which is present in corn oil? Is it saturated or nsaturated? Explain your answer

Corn oil consists of saturated fatty acids ,monounsaturated fatty acid and polyunsaturated fatty acids.But it contains a larger percentage of polyunsaturated fatty acid.To explain this, Corn oil contains 13–17% saturated fatty acids and...

1 Answers 1 views
X