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According to Hardy-Weinberg equation, p2 + 2pq + q2 = 1, where p is a frequency of the B allele, and q is a frequency of b allele. As the relative frequencies of the alleles in the population represent a percent or incidence of a certain gene variant in a population, the sum of the allele frequencies (B and b) equals 1: p + q = 1. As a result, if the relative frequency of allele B decreases, the relative frequency of allele b increases. In other words, the allele B is substituted by another allele (b) in a specific population.

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